In overall nonparametric longitudinal analysis, serum concentrations of total cholesterol, HDL, LDL, triglycerides, insulin, HOMA‐IR, HbA1c (%) (p < 0.001) and TG/HDL ratio (p < 0.05) were higher among obese than in subjects without obesity. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.