In our study, we also established a xenograft model of ESCC in BALB/C-nu/nu nude mice and found that stable low expression of SATB2 inhibits the activity of the Wnt/β-catenin signaling pathway, thus inhibiting the EMT phenotype and radioresistance of the tumor tissue, and increases apoptosis. This evidence concerns the gene SATB2 and esophageal squamous cell carcinoma.