Furthermore, NaA@SC/MAN-LPs were more effective in decreasing Acc1, Fasn, Slc27a2, and Srebf1 expression and increasing CPT1α expression compared to NaA alone, indicating that encapsulating NaA in SC and MAN dual-labeled liposomes enhanced the alleviation of hepatic lipid deposition in NAFLD mice. This evidence concerns the gene ACACA and metabolic dysfunction-associated steatotic liver disease.