TNF and colitis: In the assays designed to quantify IL-1β, IL-6, TNF-α, and the internal reference gene GAPDH in colon tissues, the outcomes indicating that all four treatment regimens reduced the expression levels of inflammatory cytokines suggested that allicin treatment, vitamin C treatment, and the combined treatment of vitamin C/allicin exerted a certain curative effect on mice with colitis, and their efficacies superseded those of the conventional immunosuppressant cyclosporin A for treating colitis.