In this study, western blotting and flow cytometric analyses indicated that panduratin A induces apoptosis by inhibiting phosphorylated EGFR (p-EGFR) and its downstream effectors, phosphorylated STAT3 (p-STAT3) and phosphorylated Akt (p-Akt), in both A549 and H1975 lung cancer cell lines. This evidence concerns the gene AKT1 and lung cancer.