Enrichment analysis showed that the top 10 signaling pathways were alcoholism, systemic lupus erythematosus, viral carcinogenesis, collecting duct acid secretion, rap1 signaling pathway, ECM–receptor interaction, PI3K-Akt signaling pathway, cell adhesion molecules, cGMP-PKG signaling pathway, and primary bile acid biosynthesis (Figure 3C). Here, AKT1 is linked to alcohol dependence.