In this study, fat emulsion and HFD significantly promoted oxidative stress (indicated by SOD, GSH, MDA, and ROS levels) and energy metabolism disorder (indicated by ATPase, as well as complex I and II levels) in liver mitochondria, and the swelling and degeneration of mitochondrial ultrastructure, which accelerated the development of MAFLD. The gene discussed is DNAH8; the disease is Disorder of energy metabolism.