Axitinib induces DNA damage response (DDR) characterized by γ-H2AX phosphorylation and Chk1 kinase activation leading to G2/M cell cycle arrest and mitotic catastrophe in glioma cell lines.Combined exposure to axitinib and bortezomib was more effective in inhibiting cell viability of all glioma cell lines. Here, H2AX is linked to glioma.