In addition, using hepatocellular carcinoma PDXs and lung cancer cases 1 and 2 PDXs, we established a system for simultaneously staining five membrane protein common cancer antigens EphB4, ROBO1, LAT1, CLDN1, GPC3, and HLA class I in six colors (Figure 5A). This evidence concerns the gene SLC7A5 and hepatocellular carcinoma.