The positive rate of GPC3 expression on the cell membrane was only 5% in hepatocellular carcinoma, 33% in hepatoblastoma, 50% in nephroblastoma: Wilms tumor, 17% in germ cell tumor, 11% in lung squamous cell carcinoma, one case of ovarian clear cell carcinoma, 8% in pharyngeal cancer, and 6% in esophageal cancer, as well as one case each of ovarian serous adenocarcinoma and lung adenocarcinoma. The gene discussed is GPC3; the disease is hepatocellular carcinoma.