Through transcriptome sequencing of three groups of TM4 cells (CTRL, OGD/R, si-H19+OGD/R), we found that the TGF-β signaling pathway, mineral absorption, alcoholic liver disease, Hippo signaling pathway, and other pathways highly enriched in KEGG all pointed to the AKT signaling pathway. Here, TGFB1 is linked to alcoholic liver diseases.