For this purpose, this study successfully established a mouse model of PD with constipation under the induction of low-dose rotenone, and, on this basis, explored the potential mechanism of PD with constipation symptoms from the perspectives of GI motility factors, the PI3K-AKT signaling pathway, intestinal inflammation and barriers, and intestinal microbiota. This evidence concerns the gene AKT1 and Constipation.