As a result, we suggest the use of a 9-color (10 antibodies) panel (CD7, CD5, sCD3, CD4, CD8, CD45, CD48, CD99, and CD16+CD56) augmented with SYTO41 as a nucleic acid dye as the most appropriate antibody set for MFC-MRD monitoring in T-ALL. The gene discussed is CD99; the disease is acute lymphoblastic leukemia.