Of note, TAI-1 specifically targets NEK2/HEC1, exhibiting minimal to no effect on a range of other kinases tested, such as CHK1/CHK2, Cdk1/Cyclin B, Aurora A/B, mTOR, PI3K, etc. TAI-1 inhibits cellular growth at the nM level for most cancer cell lines screened, including breast cancer, liver cancer, colon cancer, leukemia, and others. This evidence concerns the gene NDC80 and cancer.