To investigate the cytotoxic and antiproliferative effects of TisVed in patient-derived IDHwt and IDHmut glioma cells, we used trypan blue cytotoxicity assays with TFhigh IDHwt GBM12 cells and TFlow IDHmut BT142 cultures that have been shown by our group to have high and low TF expression, respectively [9]. This evidence concerns the gene TF and central nervous system cancer.