In combination with ChIP-seq and RNA-seq experiments, the obtained data suggest that H2AJ is a direct AR target gene, being a downstream factor in AR signaling, and partially regulates SAL-induced cellular senescence, promotes the cell growth of CSPC and CRPC cells, and inhibits the expression of mesenchymal markers, which may explain the lower expression of H2AJ in PCa metastasis. Here, H2AJ is linked to posterior cortical atrophy.