[4] In addition, NGS can provide added value to cases where finding certain allele frequencies (at least 50%) in some genes (for example, NF1, related to neurofibromatosis, p53—Li-Fraumeni, PTEN—Cowden Sd, and TSC—Tuberous Sclerosis, among others [29,30]) may be a reason for genetic testing of not only patients but also relatives. Here, NF1 is linked to tuberous sclerosis.