To further confirm the role of mitochondrial respiration in mediating the effects of WBP1, we treated CRC cells with rotenone, a well-known inhibitor of mitochondrial function that blocks electron transfer from iron-sulfur centers in mitochondrial complex I to ubiquinone (Li et al. 2003; Pereira et al. 2023; Heinz et al. 2017). This evidence concerns the gene WBP1 and colorectal carcinoma.