Then, the heat map displays the expression levels of the top 10 upregulated (PANX3, HAPLN1, UCHL1, STEAP1, COMP, EPYC, TREM2, COL10A, GJB2 and COL11A1) and downregulated (LCN2, S100A8, S100A12, HEMGN, CA1, HBD, DEFA4, MPO, CLC and LTF) genes in osteosarcoma group compared with normal group (Fig. 1B). The gene discussed is GJB2; the disease is osteosarcoma.