The expression levels of EPYC and PANX3 were significantly higher in osteosarcoma samples than in normal groups, while the trends of CD36, CLDN11 and STOM were reversed, which was consistent with results from public database (Fig. 12B and F).We further confirmed the levels of the above prognostic genes in osteosarcoma tissue samples and adjacent non-tumorous tissue by immunohistochemistry. The gene discussed is EPYC; the disease is osteosarcoma.