snRNA-seq analysis revealed a marked and progressive downregulation of RSPO3 mRNA in HSCs with advancing stages fibrosis in patients with MASLD, alcohol-associated cirrhosis or alcoholic hepatitis (Fig. 5i), whereas RSPO3 levels were low and remained largely unchanged during disease progression in ECs from the same patients (Extended Data Fig. 11i). This evidence concerns the gene RSPO3 and alcoholic hepatitis.