The mutations in Hemoglobin subunit beta (HBB_HUMAN) protein caused different diseases, for example, the residue mutation of E7V caused Sickle cell disease and E27K caused Haemoglobin E. The residue mutations of E7V and E27K triggered the different local folding variations in PFVM, which are displayed in Table 6. The gene discussed is HBB; the disease is sickle cell disease.