Immune simulations analysis demonstrated the potential of the top-ranked lead vaccine construct capability to elicit strong primary, secondary, and tertiary immune responses, with a notable increase in IFN-γ cytokine production, known for its effectiveness in combating viral diseases.75, 76 This theoretical vaccine model serves as a valuable tool for experimentalists to evaluate this model vaccine’s immunogenicity against HAstV-MLB1 infection and ultimately expedite vaccine designing strategy against this pathogen. Here, IFNG is linked to viral load.