Building on previous research demonstratingthe use of MSI forPET tracers, in this study, we demonstrate the effectiveness of usingMALDI-MSI with higher spatial resolution (50 μm) for detectingand visualizing two distinct PET tracers in the brain: UCB-J and UCB2400(Figure S1).15,16 UCB-J is aPET tracer that targets the synaptic vesicle glycoprotein 2A (SV2A),with reduced levels observed in various neurological disorders, includingepilepsy. Here, SV2A is linked to nervous system disorder.