Finally, our two-step MR analysis indicated that Lactosyl-N-palmitoyl-sphingosine (d18:1/16:0) and X-12013 have a mediating effect in the causal relationship between TNFSF14 and thyroid cancer, with mediation proportions of 8.55% and 5.78%, respectively. This evidence concerns the gene TNFSF14 and thyroid gland carcinoma.