This regulatory process was confirmed when we determined the mRNA expression levels of these enzymes in colorectal cancer cells vs. non-tumor cells where HK2, PFKFB4, aldose c (ALDOC), pyruvate kinase (PKLR), LDHA, pyruvate dehydrogenase (PDHA), SLC1A5, GLS2, GLUD1, and aspartate aminotransferase 1 (GOT1) were overexpressed (Figure 2C,D). This evidence concerns the gene GLUD1 and neoplasm.