Alterations in microbiota were analyzed in spontaneous Kras G12D expressing mouse model (KC) with pre-invasive PanIN formation, and in Kras and Trp53 expressing mouse models (KPC) with invasive PDAC development, and in an orthotopic PDAC model (using KPC-derived tumor cells) [11,17,18,20,22]. This evidence concerns the gene TP53 and neoplasm.