Namboori and co-workers (2021) [92] applied a classifier gene set of 1060 genes to separate the neurons they had obtained from ALS patient-derived hiPSC lines carrying a mutated SOD1 into distinct neural subtypes before carrying out differential gene expression analyses on these cells; as a result, these authors unveiled the activation of the TGFβ pathway in mutant MNs obtained from ALS-hiPSCs, pointing to this signaling cascade as a likely driver of gene dysregulation in ALS and MN death. Here, SOD1 is linked to amyotrophic lateral sclerosis.