BTG3 and scleroderma: Sporadic positives were observed as follows (Table 1): (1) a single mouse that received a peptide containing part of the canonical HR2 region produced modest levels of anti-centromere B autoantibodies, typically seen in some scleroderma patients (22); (2) a single mouse in the group immunized with an S1 peptide upstream of the FCS (B14) showed low levels of anti-dsDNA and anti-gliadin antibodies; and (3) one of two mice immunized with the trimer and tested by ANA had significant autoantibodies to multiple autoantigens.