HCC tumors with elevated CRSP8 and ACACA expression (Fig. 1N, bottom right) demonstrated significant cell ballooning, increased translucency, and reduced overall hematoxylin and eosin (H&E) staining compared to HCC sections with low CRSP8 and high ACACA expression (Fig. 1N, top right). This evidence concerns the gene ACACA and hepatocellular carcinoma.