Based on transcriptomic features and H&E histological morphology, we identified and annotated five tissue populations: normal glandular tissues (annotated by Krt20 and Hes1), tumor tissues (annotated by Krt14, Krt17, Hspb1 and Itgb4), muscularis tissues (annotated by Tpm2 and Myl9), submucosal tissues (annotated by Fasn, Acly, Pcx and Fabp4) and lymphoid follicular structures (annotated by Mfge8 and Ccl21a) (Fig. 1d–f; Supplementary Fig. S1a). The gene discussed is HES1; the disease is neoplasm.