To investigate the functional significance of TRAF3IP3 in the progression of human lung adenocarcinoma, we conducted stable TRAF3IP3‐overexpression A549 and PC9 cell lines and TRAF3IP3‐knockdown PC9 and H1299 cell lines through lentiviral transfections, respectively. This evidence concerns the gene TRAF3IP3 and lung adenocarcinoma.