Heterogeneity test results of IVW and MR-Egger methods demonstrated that no heterogeneity were detected, except the CCL25 levels, Bilirubin (E,Z or Z,E) levels, 5alpha-androstan-3alpha,17beta-diol monosulfate (1) levels, and bilirubin (Z,Z) to androsterone glucuronide ratio for calculus of gallbladder without cholecystitis, and the FGF-19 levels for calculus of bile duct without cholangitis or cholecystitis [Fig. S5–8, Supplemental Digital Content (http://links.lww.com/MD/O458) and Table S3–8, Supplemental Digital Content (http://links.lww.com/MD/O459)]. The gene discussed is FGF19; the disease is Cholecystitis.