Finally, 5 overlapping genes were obtained as diagnostic genes of ARDS by Venn analysis, including ACADVL, ATPase family gene 3-like 2 (AFG3L2), electron transfer flavoprotein subunit beta (ETFB), PCCB, and phosphoenolpyruvate carboxykinase 2 (PCK2) (Fig. 5E). The gene discussed is AFG3L2; the disease is acute respiratory distress syndrome.