EIF4E and cyst: Firstly, given that we observed higher levels of translation in CySCs than in cyst cells (Fig 1C and 1H), it is possible that these manipulations disrupt translation rates differently, such that eIF4F or eIF3d1 knockdown results in lower translation than other knockdowns, with a consequent effect on fate, where low translation rates lead to differentiation and high translation rates to self-renewal.