The lack of difference in Xist expression between breast cancers with and without elevated AD and lack of enrichment of DEGs on the X chromosome has a number of possible explanations: (1) lack of homogeneity in response between active and inactive X chromosomes leading to failure to meet the significance thresholds, (2) cancer-associated changes that are independent of CIZ1 expression, or (3) lack of Xi sensitivity to loss of CIZ1 assemblies possibly buffered by other repressive mechanisms. This evidence concerns the gene CIZ1 and breast carcinoma.