CIZ1 and cancer: The lack of difference in Xist expression between breast cancers with and without elevated AD and lack of enrichment of DEGs on the X chromosome has a number of possible explanations: (1) lack of homogeneity in response between active and inactive X chromosomes leading to failure to meet the significance thresholds, (2) cancer-associated changes that are independent of CIZ1 expression, or (3) lack of Xi sensitivity to loss of CIZ1 assemblies possibly buffered by other repressive mechanisms.