It has been shown that FLOT1 is an upstream regulator of the AKT/FOXO3a pathway, affecting cell proliferation in breast cancer (Lin et al. 2011) by accelerating the cell cycle, inhibiting apoptosis, and initiating EMT through the AKT, TGF‐β, and NF‐κB pathways (Cao et al. 2016, Song et al. 2012, Wei et al. 2022), which is consistent with our KEGG pathway analysis. The gene discussed is FOXO3; the disease is breast cancer.