ANXA5 and osteosarcoma: Annexin V/PI staining analysis showed that the low expression of AL031775.1 significantly increased apoptosis in MG63 and U2OS osteosarcoma cells (Figures 6K, L) Similarly, a decrease in cell proliferation and migration was observed after overexpression of AL031775.1 in the 143b osteosarcoma cell line, along with an increase in apoptosis (Figures 7A–F).