It acts as a tumor suppressor and exerts its anti-colorectal cancer effect by activating the NF-kB and JNK signaling pathways through MAP4K1 (38).Taken together, these findings suggest that the five genes we screened have demonstrated potential as tumor antigens in various cancers, making them promising candidates for the development of future mRNA vaccines. Here, NFKB1 is linked to colorectal cancer.