To further validate the up-regulation of NLRP3 levels in AD mouse model, we performed the immunofluorescence staining assay using NLRP3 antibody together with Thioflavin S (TS) staining, and found that NLRP3 immunofluorescence density were significantly increased in the DG region of hippocampus and the cortex in AD mouse, compared to the WT mouse (Figures 4A–D), suggesting NLRP3 levels significantly increase in AD mouse model brain. The gene discussed is NLRP3; the disease is Alzheimer disease.