The RT-qPCR results demonstrated that SSR4 expression was significantly elevated in ESCC cell lines (KYSE-270, TE-1, KYSE-70) compared to normal esophageal squamous epithelial cells (Het-1A), with statistical significance observed at P<0.001, P<0.01, and P<0.0001, respectively (Figure 2G). The gene discussed is SSR4; the disease is esophageal squamous cell carcinoma.