In chromatin immunoprecipitation (ChIP) assay, DNA fragments of the ITGA5 promoter containing the TFBS were detected in the eluates of KLF4 protein purified by immunoprecipitation in CRC cells, confirming that the KLF4 protein can bind to the ITGA5 promoter (Figure 3H). Here, ITGA5 is linked to colorectal carcinoma.