The cytotoxicity of L-asparaginase was tested on both normal cells (Vero cell line) and cancer cells (NFS-60, MCF-7, and HepG-2 cell lines) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) procedure. This evidence concerns the gene ASPG and cancer.