Increased memory differentiation was further validated upon in vitro re-stimulation of OT-I T cells genetically depleted for Elovl1. After 2 days of co-culture with KPC_OVA cancer cells, sgElovl1 OT-I T cells presented a higher percentage of CD44+CD62L+ memory-like T cells, whereas inhibition of mitochondrial respiration through oligomycin pretreatment, completely abrogated their memory differentiation (Fig. 7p). The gene discussed is ELOVL1; the disease is cancer.