Akin to the infection assay in ACE2 single overexpression cells, the infectivity of VLP pseudotyped virus was respectively reduced 2.5‐, 10‐, and 3.3‐fold by the S371L, S371F, and S373P mutations when infecting ACE2 + TMPRSS2 overexpressing cells (Figure 3B). This evidence concerns the gene TMPRSS2 and infection.