Thus, we used the TCGA-GBM/LGG dataset to assess the association between MFAP4 levels and ferroptosis, and showed significant associations between MFAP4 levels and ferroptosis markers including PTGS2, CHAC1, SLC40A1, TF, TFRC, GPX4, NFE2L2, HSPB1, and FTH1 (Figures 8A, B). This evidence concerns the gene SLC40A1 and glioblastoma.