miR-16-1-3p by directly targeting the 3′ UTRs, suppresses BC cell metastasis and growth, and inhibits the phosphoglycerate kinase1 (PGK1) signaling pathway, a vital part in the glycolytic pathway that catalysis transformation of 1, 3-BPG and ADP into 3-phosphoglycerate (3-PG) and ATP, of the Warburg effect. Here, PGK1 is linked to breast cancer.