Subsequently, all factors identified from the univariate analysis were included in the multivariate analysis: age, PDCD1/CD27 ratio, PDCD1/TNFSF4 ratio, IDO1/TMIGD2 ratio, IDO1/TNFSF4 ratio, neoadjuvant treatment, initial weight of tumor, ER status, PR status, HER2 status, and TNM stage. This evidence concerns the gene ESR1 and neoplasm.