Piezo1 is one of the major ion channels conferring mechanosensitivity to GBM stem cells, and RNA of Piezo1 knockdown glioblastoma cell lines sequencing and dual-assay TCGA database analysis of Piezo1-related genes showed that Piezo1 is associated with activation of extracellular matrix (ECM), actin cytoskeleton remodeling and integrin adhesion signaling. Here, PIEZO1 is linked to glioblastoma.