The combination of TBMS1 and TMZ strongly induces apoptosis in glioblastoma cells, accompanied by the formation of strong DSB, γH2AX foci, and an increase in PARP cleavage, and reduces the expression of MGMT in TMZ-resistant GBM cells, thereby reducing DNA damage repair and synthetic functions and restoring the sensitivity of MGMT + cells to TMZ. The gene discussed is MGMT; the disease is glioblastoma.