LGALS4 and neoplasm: Moreover, we found no evidence of sporadic genomic loss the tubGal80 insertion in males because: (a) Notch protein expression, regardless of its variability in intensity, was detectable in essentially all ISCts-Gal4 and esgts-Gal4, GFP positive cells (Figures S8A and S8B), and (b) GFP de-repression in ISCs and EBs was very frequent, and cells exhibited size variation (Figures S8A and S8B), which is unlike the rare and uniform ISC tumor cells that accompany the spontaneous loss of Notch signaling in old males.36