These data suggest that VSV-eGFP can promote depletion of hSpt16 that is SUMOylated with either SUMO-1 or SUMO-2/3 and that the specific loss of hSpt16SUMO levels during VSV-eGFP infection is not due to a generalized decrease in intracellular SUMOylation. Here, SUMO1 is linked to infection.