To study the role of nuclear PDGFB in glioma progression, we developed a new model of immunocompetent glioma using in utero electroporation (IUE) of piggyBac transposase driving GFP and PDGFB overexpression (PDGFBwt) in neural progenitor cells (Figs. S1e–f). The gene discussed is PDGFB; the disease is glioma.